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KMID : 0043319960190040264
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Archives of Pharmacal Research
1996 Volume.19 No. 4 p.264 ~ p.268
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Effect of Ginseng Saponin on Gap Junction Channel Reconstituted with Connexin 32
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Hong Eun-Jung
Huh Keun
Rhee Seung-Keun
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Abstract
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Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an established in vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1 % (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.
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KEYWORD
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Ginseng, Gap junciton channel, Connexin32, Sucrose-permeability, Tiriton X-100, Reconstitution
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